CSR Biotech MI-SIM

MI-SIM (Machine Intelligent-Structured Illumination Microscope) represents a comprehensive super-resolution microscopy solution for live cell imaging, entirely conceived, developed, produced, and marketed by CSR Biotech. This advanced technology was born from the collaborative efforts of an interdisciplinary team at Peking University, renowned for their groundbreaking work in biomedical imaging. As a tool of choice, MI-SIM offers exceptional capabilities for observing live cell dynamics with ultra-high spatial and temporal resolution.

With its exceptional spatial resolution surpassing 60 nm, swift imaging capabilities reaching up to 1500 frames per second, and an expansive field of view (FOV) of 150 μm with a 100 × objective, the MI-SIM sets new standards in 3D super-resolution imaging of various dynamic processes in living cells. Notably, MI-SIM's advanced sensitivity and reduced photo-toxicity facilitate prolonged imaging sessions while minimizing photobleaching effects.

MI-SIM boasts a suite of sophisticated features, including real-time super-resolution, multi-camera and multi-site imaging capabilities, and intelligent region-of-interest (ROI) planning. It also excels in stitching within large FOV, 3D real-time tracking, multimodal focusing, and event-driven tracking. These capabilities underscore MI-SIM's versatility, ease of use, and user-centric design, reflecting CSR Biotech's leadership in the realms of advanced imaging, online decision-making, and intelligent control systems.

Highlights 

1. 2D/3D Realtime Super Resolution - First to realize real-time SR imaging, seeing is obtaining

The Real-time SR helps to rapidly localize the distribution of cristae within mitochondria.

2. 60 nm Super Resolution - First to achieve 60 nm resolution in live-cell imaging with extremely low photobleaching

First in the world to capture the 60 nm ring of NUP98 in a live cell and its dynamics

3. Long-term Live-cell Tracking - World's 1st to realize real-time intelligent tracking for long-term live cell imaging

Long-term tracking of protein and mitochondria dynamics during U2OS cell apoptosis while maintaining the center of the field of view unchanged and in focus

Key Benefits

1. Next–generation SIM resolution: With data showing 60 nm resolution in X–Y and 200 nm in Z, MI–SIM enables researchers to resolve fine subcellular structures beyond the reach of diffraction-limited microscopy systems.

2. Designed for live samples: 2D-SIM imaging in live U2OS cells reveals dynamic interactions between mitochondria (cyan) and the endoplasmic reticulum (yellow), enabling rapid visualization of organelle behavior and direct observation of contact sites between different organelles.

3. Multiple imaging modes: 2D, 3D and TIRF SIM options give researchers flexibility across different imaging workflows.

Dual camera module 

SYNER dual camera module

MI–SIM offers the option to include the SYNER dual camera module, supporting high-speed sequential or simultaneous dual-channel SIM acquisitions. SYNER includes simple digital image alignment tools, helping maintain consistency across channels. Unlike many dual camera systems, SYNER does not limit workflows to two fixed channels. With dual filter wheels and motorized dichroic switching, users can move between single-camera bypass mode and high-speed multi-channel acquisitions with more than four channels.

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